Your 12-Pette
®
Pipettor has been calibrated and verified for
accuracy by the Corning Q.C. laboratory and unless severely
mistreated should never need recalibration. A quality control
statement showing C.V. and accuracy of your individual
instrument is packaged with your 12-Pette pipettor.
Unit Serial number .
All parts of the 12-Pette pipettor are made of high strength
engineering materials to give you many years of reliable serv-
ice; however, it should be treated with the same care you
would give any other precision instrument such as a camera or
microscope. The case provided makes a logical place to keep
and protect your 12-Pette pipettor. For long periods of stor-
age set the volume at 200 µL. Then, before using, make sever-
al full strokes of the dispensing trigger.
There are no serviceable parts inside the 12-Pette pipettor
and you should not attempt to take it apart. It cannot be re-
assembled to perform accurately without the special calibra-
tion instruments and fixtures used by Corning. Should your
12-Pette Pipettor ever fail to operate properly, call Technical
Service at 1.800.492.1110 or 1.978.442.2200 for help.
Setting the Volume
Corning has chosen a micrometer head design for setting
volume. This is an extremely accurate method of adjustment
since volumes can be precisely set, line on line, and fractional
values can be estimated on the analog scale to about one tenth
of a milliliter.
The following instructions are offered for those not familiar
with the use of a micrometer:
A. The fixed scale on the inner barrel shows volumes in units
of ten µL, i.e. 20 µL, 30 µL, 50 µL, etc.
B. To set a volume of 100 µL, turn the micrometer head using
the quick setting knob until you can read 100 but not 110
on the fixed scale on the inner barrel. Now, holding the
micrometer head, line up 0 on the moveable scale with the
horizontal index line on the inner barrel. If set correctly,
you now can read 100 and all smaller volumes on the fixed
scale. You can also see the index line to the left of 100, but
the 110 index line is covered by the edge of the micrometer
head.
C. Now rotate the head so that 1, 2, and 3 appear in order.
Line up 3 with the index line. Volume indicated is 100 plus
3 or 103 µL.
D. Now rotate the head to the line halfway between 3 and 4.
Volume is 100 plus 3 µL, or 103 µL or 103.5 µL. The
setting increments are very large on the micrometer head
to allow easy reference to volume settings. (See Figure 1.)
Pipette Tips
Your 12-Pette pipettor has been designed and calibrated with
Corning
®
tips. They are available in bulk or in racks of 96,
spaced for picking up 8 or 12 at one time on the tip holders
of your 12-Pette pipettor.
To eject tips, hold the 12-Pette pipettor vertically over a suit-
able waste container and pull ejector trigger firmly. (Ejector
trigger is remote from dispensing trigger to avoid accidental
ejection.)
For more information about Corning tips, visit
www.corning.com/lifesciences.
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